Breast Cancer by Jian Cao

Author:Jian Cao
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

11.Measure fluorescence versus time for 15 min and then find the slope over the desired period (typically between 6 and 10 min).

12.If the stock concentration is too high, dilute the enzyme with the 25 % glycerol solution (see Note 6).

13.Repeat steps 3–12 until the slope of the enzyme is approximately 30–35.

14.Aliquot diluted enzyme into 12 μL aliquots and store at −80 °C (see Note 7).

3.3 Inhibitor Dilution

1.Remove 40 μL inhibitor stock from storage and dilute with 40 μL of appropriate solvent, such as DMSO (see Note 8).

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